Which instruction is involved in the Navy's physical fitness standards?

The instruction that outlines the Navy's physical fitness standards is OPNAVINST 6100.1. This instruction provides the framework for the physical readiness program within the Navy, detailing requirements and standards for physical fitness testing, as well as the necessary guidelines for maintaining and improving physical readiness among sailors. OPNAVINST 6100.1 emphasizes the Navy's commitment to ensuring personnel are physically fit to meet the demands of their duties and ultimately enhance mission readiness.

While other options may pertain to various aspects of Navy policy, training, or wellbeing, OPNAVINST 6100.1 specifically addresses the criteria and procedures for physical fitness assessments, making it the correct reference in this context.